DIGESTION & COLON
Enzymes are important for the body's proper absorption and utilization of food. Over time, the body's ability to make certain enzymes reduces as part of the natural aging process. Many
scientists now believe that maintaining normal levels of key enzymes is important to maintaining overall health. Enzymes are responsible for every activity of life. Digestive and metabolic are
the two primary classifications of enzymes within the body. Proteases (aids in digesting protein), amylases (aids in digesting carbohydrates), and lipases (aids in digesting fats) are the
three primary digestive enzymes, which function as the biological catalyst to breaking down food. In today's world of processed and fast foods, the body must work harder to break down
food and absorb the nutrients. Poor eating habits, such as improper or inadequate chewing and eating on the run contribute to reduced levels of digestive enzymes. In addition to a
reduction in essential enzymes, poor eating habits, some medications and illness deplete the body's probiotics, which are necessary for maintaining a healthy digestive tract and promoting
a healthy immune system. Isotonix® Digestive Enzyme Formula with Probiotics is an isotonic-capable food supplement that is made from a combination of DigeZyme®, a blend of amylase,
protease, cellulase, lactase, and lipase, and Lactospore®, the probiotic Lactobacillus sporogenes, and potassium, magnesium, maltase and sucrase, designed to replenish essential
digestive enzymes and probiotics, contributing to good digestive health. Isotonix Digestive Enzyme Formula helps maintain healthy cholesterol levels, promotes digestion and absorption of
nutrients, supports a healthy digestive tract, supports a healthy immune system and may help ease occasional stomach upset. DigeZyme® is a multi-enzyme complex consisting mainly of
amylase (starch hydrolyzing enzyme), protease (protein hydrolyzing enzyme) and lipase (fat hydrolyzing enzymes). In addition to these, it also contains cellulase (that hydrolyzes cellulose)
and lactase (that hydrolyzes lactose). The enzymes in this complex are of microbial origin (fungal amylase, lipase, lactase, cellulase; and a bacterial neutral protease). The product is
therefore entirely of non-animal origin. Lactospore®, a preparation containing viable spores of L. sporogenes, L. sporogenes preparations have been used in successful clinical trials in the
management of gastrointestinal issues, mild lactose intolerance and supporting healthy immune functions. *These statements have not been evaluated by the Food and Drug
Administration. This product is not intended to diagnose, treat, cure or prevent any disease.

Benefits:
The best performing digestive enzyme supplement on the market today!
Contains the patented ingredient Digezyme® which:
Promotes digestion
Provides enzymes & good bacteria that promotes the absorption of nutrients
Supports a healthy digestive tract
Supports a healthy immune system
Convenient on-the-go packets
Additional Benefits -
Offers a unique blend of digestive enzymes & probiotics
Helps maintain healthy cholesterol levels
May help ease occasional stomach upset
Gluten Free - Vegetarian - No Detectable GMO - No Fillers - Drinkable


INGREDIENTS:
Amylase (2400U*)
Amylases are enzymes that catalyze the hydrolysis of alpha-1, 4-glycosidic linkages of polysaccharides to yield dextrins, oligosaccharides, maltose and D-glucose. Amylases are derived
from animal, fungal and plant sources. Pancreatin and pancrelipase contain amylase derived from the pancreas of animals, usually porcine pancreas. Amylase is also derived from barley
malt and the fungus Aspergillus oryzae. There are a few different amylases. These enzymes are classified according to the manner in which the glysosidic bond is attacked. Alpha-amylases
hydrolyze alpha-1, 4-glycosidic linkages, randomly yielding dextrins, oligosaccharides and monosaccharides. Alpha-amylases are endo-amylases. Exoamylases hydrolyze the alpha-1, 4-
glycosidic linkage only from the non-reducing outer polysaccharide chain ends. Exoamylases include beta-amylases and glucoamylases (gamma-amylases, amyloglucosidases). Beta-
amylases yield beta-limit dextrins and maltose. Gamma-amylases yield glucose. Amylases are used as digestants. Amylase activity is expressed as Dextrinizing Units or DU.

Protease (600U)
Proteases (proteinases, peptidases or proteolytic enzymes) are enzymes that break peptide bonds between amino acids in proteins. The process is called proteolytic cleavage, a common
mechanism of activation or inactivation of enzymes especially involved in blood coagulation or digestion. They use a molecule of water for this and are thus classified as hydrolases.

Proteases occur naturally in all organisms and constitute one to five percent of the gene content. These enzymes are involved in a multitude of physiological reactions from simple digestion
of food proteins to highly regulated cascades (e.g. the blood clotting cascade, the complement system, apoptosis pathways and the invertebrate prophenoloxidase activating cascade).
Peptidases can break either specific peptide bonds (limited proteolysis), depending on the amino acid sequence of a protein, or break down a complete peptide to amino acids (unlimited
proteolysis). The activity can be a destructive change abolishing a protein's function or digesting it to its principal components, an activation of a function or a signal in a signaling pathway.

Lactase (400U)
Lactase (LCT), a member of the galactosidase family of enzymes, is involved in the hydrolysis of the disaccharide lactose into constituent galactose and glucose monomers. In humans,
lactase is present predominantly along the brush border membrane of the differentiated enterocytes lining the villi of the small intestine.

Lactase is essential for digestive hydrolysis of lactose in milk. Deficiency of the enzyme causes lactose intolerance; most humans become lactose intolerant as adults.

Lactase has an optimum temperature of about 48° C for its activity and an optimum pH of 6.5. In humans, the gene is localized on the second chromosome (2q21). Bacterial and Archaea
lactase lacks a membrane binding domain and free float around the cell; these also tend to be more general -galactosidase that will cleave more than just lactose.

Lipase (100U)
A lipase is a water-soluble enzyme that catalyzes the hydrolysis of ester bonds in water-insoluble, lipid substrates. Most lipases act at a specific position on the glycerol backbone of a lipid
substrate (A1, A2 or A3). In the example of human pancreatic lipase (HPL), which is the main enzyme responsible for breaking down fats in the human digestive system, a lipase acts to
convert triglyceride substrates found in oils from food to monoglycerides and free fatty acids. A myriad of other lipase activities exist in nature, especially when the phospholipases and
sphingomyelinases are considered.

Lipases are ubiquitous throughout living organisms, and genes encoding lipases are even present in certain viruses. While a diverse array of genetically distinct lipase enzymes are found
in nature, most are built on an alpha/beta hydrolase fold and employ a chymotrypsin-like hydrolysis mechanism involving a serine nucleophile, an acid residue (usually aspartic acid), and a
histidine.*

Some lipases work within the interior spaces of living cells to degrade lipids. In the example of lysosomal lipase, the enzyme is confined within an organelle called the lysosome. Other lipase
enzymes, such as pancreatic lipases, are found in the spaces outside of cells and have roles in the metabolism, absorption and transport of lipids throughout the body. As biological
membranes are integral to living cells and are largely composed of phospholipids, lipases play important roles in cell biology. Furthermore, lipases are involved in diverse biological
processes ranging from routine metabolism of dietary triglycerides to cell signaling and inflammation. Several different types of lipases are found in the human body, including pancreatic
lipase, hepatic lipase, lysosomal lipase, gastric lipase, endothelial lipase and as various phospholipases.*

Cellulase (20U)
Cellulase is an enzyme complex which breaks down cellulose to beta-glucose. It is produced mainly by symbiotic bacteria in the ruminating chambers of herbivores. Aside from ruminants,
most animals (including humans) do not produce cellulase in their bodies, and are therefore unable to use most of the energy contained in plant material.

Enzymes which hydrolyze Hemicellulose are usually referred to as hemicellulase and are usually classified under cellulase in general. Enzymes that cleave lignin are occasionally classified
as cellulase, but this is usually considered erroneous.
Cellulase is an enzyme derived from the fungi Aspergillus niger and Trichoderma longbrachiatum or other sources. Cellulose is an indigestible plant polysaccharide. It is the principal
constituent of the cell wall of plants. Cellulase has cellulolytic activity, meaning that it hydrolyzes cellulose. Cellulase hydrolyzes the beta-D-1, 4-glycosidic bonds of cellulose. Cellulase
derived from Trichoderma longbrachiatum is comprised of an enzyme complex consisting of cellulase, a glucosidase, cellobiohydrolase and a glucanase. This complex converts cellulose to
beta-dextrins and ultimately to D-glucose. Cellulase is used as a digestive aid, particularly in animals, and for the management of flatulence. The activity of cellulase is expressed in
cellulose units or CU.

Cellulase is used for commercial food processing in coffee. It performs hydrolysis of cellulose during drying of beans. Cellulase is used in the fermentation of biomass into biofuels, although
this process is relatively experimental at present. Cellulase is used to address Phytobezoars, a form of cellulose bezoar found in the human stomach.

Maltase (125 MWU*)
Maltase is one enzyme produced by the cells lining the small intestine to break down disaccharides. It comes under the enzyme category carbohydrase (which is a subcategory of
hydrolase), and the disaccharide it hydrolyses is maltose.

Maltase is secreted by the surface cells of the villi, which are thin projections on the mucosa. These are found throughout the small intestine, but differ in shape in the duodenum and ileum
sections.

The maltase works like any other enzyme, with the substrate (maltose) binding with the active site. When the maltose has bonded with the maltase, the former is hydrolysed, split into its
component parts (i.e. two molecules of glucose.) This is done by breaking the glycosidic bond between the 'first' carbon of one glucose bond, and the 'fourth' carbon of the other (a 1-4
bond).

Sucrase (400 SU*)
Sucrase is the enzyme involved in the hydrolysis of sucrose to fructose and glucose. It is secreted by the tips of the villi of the epithilum in the small intestines. Its levels are reduced in
response to villi blunting events such as ciliac sprue. Sucrase increases during pregnancy and lactation as villi hypertrophy.*

Magnesium (Carbonate) (24 mg)
Foods rich in magnesium include unpolished grains, nuts and green vegetables. Green leafy vegetables are good sources of magnesium because of their chlorophyll content.  Meats,
starches and milk are less rich sources of magnesium. Refined and processed foods are generally quite low in magnesium. The average daily magnesium intake in the U.S. for males nine
years and older is estimated to be about 323 milligrams; for females nine years and older, it is estimated to be around 228 milligrams. Some surveys report lower intakes, and some believe
that the dietary intake may be inadequate for many.

Magnesium is a component of the mineralized part of bone and is necessary for the metabolism of potassium and calcium in adults.  It helps maintain normal levels of potassium,
phosphorus, calcium, adrenaline and insulin.It's also important for the mobilization of calcium, transporting it inside the cell for further utilization.  It plays a key role in the functioning of
muscle and nervous tissue. Magnesium is necessary for the synthesis of all proteins, nucleic acids, nucleotides, cyclic adenosine monophosphate, lipids and carbohydrates.This mineral
also helps maintain healthy kidneys and bladder.  Further, magnesium helps indirectly in combating oxidative stress and lipid peroxidation involved with the aging process.

Magnesium is required for release of energy, regulation of the body temperature, proper nerve function, helping our bodies handle stress and regulating our metabolism. Magnesium works
together with calcium to regulate the heart and blood pressure. Importantly, magnesium is also required by your body to build healthy bones and teeth, and is required for proper muscle
development.It works together with calcium and vitamin D to help keep bones strong.

Potassium (Bicarbonate) (88 mg)
Foods rich in potassium include fresh vegetables and fruits such as bananas, oranges, cantaloupe, avocado, raw spinach, raw spinach, cabbage and celery.

Potassium is an essential macromineral that helps to maintain fluid balance.  It also plays a role in a wide variety of biochemical and physiological processes.Among other things, it is
important in the transmission of nerve impulses, the contraction of cardiac, skeletal and smooth muscle, the production of energy, the synthesis of nucleic acids, the maintenance of
intracellular tonicity and the maintenance of normal blood pressure. Potassium promotes normal muscle relaxation and insulin release.It also promotes normal glycogen and protein
synthesis.Potassium is an electrolyte that promotes proper heartbeat.Potassium is also important in releasing energy from protein, fat and carbohydrates during metabolism.

Potassium also regulates water balance and supports the body's normal recuperative powers. Potassium promotes joint health and comfort.Potassium is crucial for the elimination of
wastes. Potassium promotes head comfort, promotes faster healing of cuts, bruises and other minor injuries and generally contributes to a sense of well-being. Potassium is stored in the
muscles.


Lactobacillus sporogenes - Lactospore®*** (150,000,000 CFU**)
Lactobacillus sporogenes is a lactic acid bacillus preparation manufactured and distributed by the SABINSA CORPORATION.

The foundations of probiotic (meaning "in favor of life") microbiotherapy lie in the postulate of Metchnikoff, a Russian physician, that the growth of toxin-producing putrefactive organisms in
the gastrointestinal tract could be controlled by the implantation of beneficial lactobacilli in the gut. The clinical application of preparations containing lactobacilli was initiated on the basis of
Metchnikoff's Theory of Longevity, which associates with prolonged youthfulness and a healthy old age with the continuous ingestion of lactobacilli. Metchnikoff attributed the longevity of
the residents of the Balkan countries to the regular consumption of Bulgarian buttermilk.  In the early 1900's, he claimed to have successfully cured many of his patients who suffered from
a wide variety of organic illnesses, ranging from dry skin to gastrointestinal disorders, through the therapeutic use of Lactobacilli. Metchnikoff suggested that aging is the process of chronic
putrefactive intoxication caused by certain intestinal bacteria and that these harmful effects could be mitigated through regular ingestion of live Lactobacillus cultures - a postulate that
created a sensation in those early days. The enthusiasm shown then by eminent doctors of that time, advocating the therapeutic use of Lactobacillus, laid the foundations of lactobacillus
therapy or microbiotherapy.

Fermented milks have been a part of the human diet since ancient times. Their efficacy in alleviating gastrointestinal disorders has been exploited in systems of traditional medicine the
world over. Lactic acid bacteria, the indigenous microbial flora in fermented milks and natural inhabitants of the human gastrointestinal tract were thought to be responsible for the longevity
of their hosts through their curative and prophylactic actions.

The role of lactic acid bacteria in gastrointestinal microecology has been the subject of extensive research. It is widely believed that these bacteria prevent the growth of putrefactive
microorganisms responsible for ill health by competitive inhibition, the generation of a non-conducive acidic environment and/or by the production of bacteriocins. Their metabolites may
include B group vitamins. Their proteolytic, lipolytic and beta-galactosidase activities promote the digestibility and assimilation of ingested nutrients, thereby rendering them valuable in
convalescent/ geriatric nutrition.Lactic acid bacteria also colonize the skin and mucus membranes and promote skin and urinary tract health.Lactobacilli promote vaginal health.They utilize
glycogen in the vaginal epithelial cells to produce lactic acid which helps to maintain the pH of this environment between 4.0 and 4.5, which creates a healthy environment.  

*U, MWU, SU = Units of enzyme activity
**CFU = Colony forming units

ENZYMES & PROBIOTICS COLON
HEALTH